pge 2 elisa kit  (R&D Systems)

Journal: bioRxiv

Article Title: Actionable spatial prostanoid barriers constrain BiTE-driven adoptive T cell immunity in intact human tumors

doi: 10.64898/2026.03.26.713601

Figure Lengend Snippet: A, Basal PGE 2 quantified in NSCLC PDE supernatants (pg/mL), shown as a heatmap illustrating inter-lesion variability. B, Correlation between basal PGE 2 and MSLN-BiTE-induced CD8+ T cell activation in NSCLC PDEs, expressed as FC in CD8 + CD137 + frequencies relative to UT CTRL (Holm-corrected P value shown). C, Correlation between basal PGE 2 and MSLN-BiTE-induced granzyme B release in NSCLC PDEs, expressed as log2 FC relative to UT CTRL per patient (r and P value shown). D, COX blockade rescue experiment in two NSCLC cases (Lung 31 and Lung 33): PDEs were pretreated with ketorolac (COXi, 1 µM) for 1 h prior to co-culture with MSLN-BiTE T cells, and CD8 + CD137 + activation together with granzyme B, CXCL9, and CXCL10 were quantified as FC over UT CTRL. E, Pharmacodynamic verification of prostanoid pathway inhibition in the HGSOC validation cohort (n = 11), showing PGE 2 levels at baseline and after COXi (ketorolac, 1 µM) or EP2/EP4 inhibition (EP2/4i, 1 µM). F, CD8 + T cell activation in HGSOC PDE co-cultures, reported as %CD8 + CD137 + among CD8 + cells across conditions (condition matrix shown). G-H, FLAG-based discrimination of engineered versus bystander/resident CD8 + T cells in HGSOC PDEs, showing frequencies of CD8 + CD137 + FLAG + exogenous cells ( G ) and CD8 + CD137 + FLAG - bystander/ endogenous cells ( H ). (I) IFNγ secretion in HGSOC PDE co-cultures, expressed as FC over UT CTRL across conditions. ( J ) Cytokine and chemokine coordination across HGSOC PDEs visualized as Pearson correlation matrices for untreated, MSLN-BiTE, MSLN-BiTE + COXi, and MSLN-BiTE + EP2/4i conditions (circle size indicates correlation magnitude; color indicates direction). K, Myeloid polarization in HGSOC PDEs quantified as %CD68 + CD80 + among CD68 + macrophages across conditions. ( L-N ) TNFα ( L ), GM-CSF ( M ), and IL-10 ( N ) secretion in HGSOC PDE co-cultures, expressed as FC over UT CTRL. Boxplots show median and interquartile range with whiskers as displayed; each dot represents one patient. For HGSOC panels ( E-N ), statistics were performed on patient-level values using one-way repeated-measures ANOVA with multiple-comparisons correction as indicated (GraphPad Prism v10.1.2). Figure generated with BioRender.

Article Snippet: Basal prostaglandin E2 levels in culture supernatants from PDEs were quantified using a PGE 2 ELISA kit (Cayman Chemical, 514010) according to the manufacturer’s instructions.

Techniques: Activation Assay, Co-Culture Assay, Inhibition, Biomarker Discovery, Generated

Journal: Nature Communications

Article Title: Gut microbiota-derived butyrate primes systemic immunity in honey bees by mediating lipid metabolic reprogramming

doi: 10.1038/s41467-026-69073-0

Figure Lengend Snippet: a PCA of abdominal metabolomes from GF and butyrate-supplemented bees at day 5 post-supplementation (n = 6 per group, each point represents one biological replicate). b Heatmap of the discriminating metabolites in the arachidonic acid pathway in the abdomen of GF and butyrate-supplemented bees. c Prostaglandin E 2 (PGE 2 ) levels in abdomen, hindgut and hemolymph in GF, CV, CL, and butyrate-supplemented bees at day 5 post-colonization (abdomen: n = 6; hindgut and hemolymph: n = 4; each pooled from 5 individuals from 3 cup cages). One of two independent experiments (Supplementary Fig. ). Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. d Knockdown of fat body Pla2 in GF bees following dsRNA injection (GFP dsRNA as control). n = 8 per group from 3 cup cages. P = 0.0394, two-sided Welch’s t test. e Effect of Pla2 RNAi on PGE 2 levels in fat bodies of GF and butyrate-supplemented bees ( n = 5 replicates per group, each pooled from 5 individuals from 3 cup cages). P = 0.0039, two-sided Student’s t test. f Survival of GF, GF + PGE 2 , CL, and CL+acetylsalicylic acid (ASA) bees post- Hafnia infection. Bars represent mean ± s.d. from three replicates per group (30 individuals per replicate) from one of two independent experiments (Supplementary Fig. ). Two-sided Mantel–Cox test; different letters indicate significant differences ( P < 0.05). g AMP expression in fat bodies before and 3 h post- Hafnia infection (GF as control; n = 8 per group from 3 cup cages. Two-sided Mann–Whitney and two-sided unpaired Student’s t test, depending on data normality. h In vivo hemocyte staining using CM-DiI (red) before infection (3 h after PGE 2 supplementation) and at 3 h post-infection with GFP- Hafnia (green). Dorsal vessels were outlined with white dotted lines. Scale bars: 250 μm. Representative images from two independent experiments (Supplementary Figs. , ). In ( c – e , g ), bars represent mean ± s.e.m. * P < 0.05, ** P < 0.01, and *** P < 0.001. All P values are listed in Supplementary Dataset . Source data are provided as a Source Data file.

Article Snippet: The PGE 2 levels were measured using a PGE 2 ELISA kit (Cat#D751014, Sangon Biotech, China) according to the manufacturer’s protocol.

Techniques: MANN-WHITNEY, Knockdown, Injection, Control, Infection, Expressing, In Vivo, Staining

Journal: bioRxiv

Article Title: Dietary cholesterol reduces blood pressure and alters lipid profiles in stroke-prone spontaneously hypertensive rats

doi: 10.64898/2026.01.27.702179

Figure Lengend Snippet: (A) Hepatic and (B) renal PGE 2 levels. Values are presented as mean ± SD (n = 6/group). * p < 0.05 vs. Ctr. Abbreviations: Ctr, control group; Chol, cholesterol group; Stt, statin group; SD, standard deviation; PGE 2 , prostaglandin E 2 ; SHRSP, stroke-prone spontaneously hypertensive rats.

Article Snippet: The concentrations of prostaglandin E 2 (PGE 2 ) in the liver and kidneys were evaluated using the PGE 2 Monoclonal ELISA Kit (Cayman Chemical Co., Ann Arbor, MI).

Techniques: Control, Standard Deviation